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Articles
Published: 2020-12-24

Infectious serology, how to test large series without pooling samples

Department of Hemobiology EHU "1er Novembre 1954" Oran, Algeria
Department of Hemobiology EHU "1er Novembre 1954" Oran, Algeria
Faculty of Medicine University of Oran 1 Ahmed Ben Bella, Algeria
Department of Hemobiology CHU Oran, Algeria
ELISA, pooling sera, infectious serology, Algeria

Abstract

Pooling samples for serological testing was used first during the second world war. It was described later as a cost-effective technique permitting large screening of populations, especially for new infectious diseases. However, the dilution effect is responsible for decreasing sensitivity, limiting its use in practice, especially in blood banking. In this paper, we describe a modification of the classic enzyme-linked immunosorbent assay (ELISA) procedure, which permits the test of indefinite samples using just one well. Specimens are tested pure one by one without any dilution, so sensitivity remains unchanged. This new procedure is time-consuming but can be considered as a revolution in qualitative ELISA testing.



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How to Cite

1.
Bennaoum MN, Adda A, Chekkal M, Seghier F. Infectious serology, how to test large series without pooling samples. jidhealth [Internet]. 2020 Dec. 24 [cited 2024 Mar. 28];3(Special2):276-7. Available from: https://jidhealth.com/index.php/jidhealth/article/view/87