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Articles
Published: 2023-12-02

Detection of SARS-CoV-2 variants among travelers crossing the northern international border checkpoint in Duhok province, Iraq

Department of Pharmacology, College of Pharmacy and Department of Biology, College of Science, University of Duhok, Iraq
Biology Department, College of Science, University of Duhok, Duhok, Iraq
Medical Laboratory Technology Department, College of Health and Medical Techniques-Shekhan, Duhok Polytechnic University, Duhok, Iraq
Preventive Health Affairs Directorate, Directorate General of Health, Duhok, Iraq
Department of Physiology, Biochemistry, and Pharmacology, College of Veterinary Medicine, University of Mosul, Mosul, Iraq; College of Nursing, The American University of Kurdistan, Duhok, Iraq
Department of Clinical Pharmacy, College of Pharmacy, University of Duhok, Duhok, Iraq.

Abstract

Background: Variants of SARS-CoV-2 have been detected along with the worldwide COVID-19 transmission. Insufficient data exists regarding SARS-CoV-2 variants in Iraq, particularly concerning travelers at Iraqi border crossings. This study aimed to screen SARS-CoV-2 variants among individuals entering Iraq from Turkey through the Ibrahim Khalil international border checkpoint. 

Methods: A total of 116 (52 females, 64 males) COVID-19-positive cases from June 6, 2021, to July 30, 2021, were randomly selected from the Ibrahim Al-Khalil border crossing point between Turkey and Iraq. The patients were Iraqis tested positive for COVID-19 through nasopharyngeal and oropharyngeal swab collections. The confirmation of SARS-CoV-2 cases involved RNA extraction and real-time reverse transcription polymerase chain reaction (RT-PCR), following the World Health Organization guidelines. All samples were subjected to variant detection using the reverse transcription RT-PCR method. The RNA was extracted using a Zybio nucleic acid extraction kit and the RT-PCR was conducted using SARS-CoV-2 S-gene mutation detection RT-PCR kit.

Results: In the 116 patients, we detected variants B.1.1.7, B.1.617.1.2, B.1.617.1.3, and Omicron sub-lineages (B.1.1.529, BA.1) at frequency rates of 13, 16, 45, and 36 cases, respectively. The highest prevalence rate was the delta variant among the tested subjects (38.8%) followed by the Omicron sub-lineages (B.1.1.529, BA.1) at 31%.

Conclusion: This finding, which is the first of its kind at the northern Iraqi border checkpoint, suggests that these two variants could be a source of COVID-19 circulating in the Duhok province in Iraq. Additional studies are warranted across the country’s border entry points.



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References

  1. Martín Sánchez FJ, Martínez-Sellés M, Molero García JM, et al. Insights for COVID-19 in 2023. Rev Esp Quimioter. 2023;36(2):114-124. doi: 10.37201/req/122.2022.
  2. Hadj Hassine I. Covid-19 vaccines and variants of concern: A review. Rev Med Virol. 2022;32(4): e2313. doi: 10.1002/rmv.2313.
  3. Long B, Carius BM, Chavez S, et al. Clinical update on COVID-19 for the emergency clinician: Presentation and evaluation. Am J Emerg Med. 2022; 54:46-57. doi: 10.1016/j.ajem.2022.01.028.
  4. Vasireddy D, Vanaparthy R, Mohan G, et al. Review of COVID-19 variants and COVID-19 vaccine efficacy: what the clinician should know? J Clin Med Res. 2021;13(6):317-325. doi: 10.14740/jocmr4518.
  5. Ramesh S, Govindarajulu M, Parise RS, et al. Emerging SARS-CoV-2 variants: a review of its mutations, its implications and vaccine efficacy. Vaccines (Basel). 2021;9(10):1195. doi: 10.3390/vaccines9101195.
  6. Merza MA, Almufty HB. The Omicron variant is striking Iraqi Kurdistan in January 2022: would preventive measures contain the new wave? Disaster Med Public Health Prep. 2022;9: 1-2. doi: 10.1017/dmp.2022.156.
  7. Merza MA, Haleem Al Mezori AA, Mohammed HM, et al. COVID-19 outbreak in Iraqi Kurdistan: the first report characterizing epidemiological, clinical, laboratory, and radiological findings of the disease. Diabetes Metab Syndr. 2020;14(4):547-554. doi: 10.1016/j.dsx.2020.04.047
  8. Al-Rashedi NAM, Alburkat H, Hadi AO, et al. High prevalence of an alpha variant lineage with a premature stop codon in ORF7a in Iraq, winter 2020-2021. PLoS One. 2022;17(5): e0267295. doi: 10.1371/journal.pone.0267295
  9. Sabir DK. Analysis of SARS-COV2 spike protein variants among Iraqi isolates. Gene Rep. 2022; 26:101420. doi: 10.1016/j.genrep.2021.101420.
  10. Grépin KA, Aston J, Burns J. Effectiveness of international border control measures during the COVID-19 pandemic: a narrative synthesis of published systematic reviews. Philos Trans A Math Phys Eng Sci. 2023;381(2257):20230134. doi: 10.1098/rsta.2023.0134.
  11. Salih DA, Ahmed JQ, Qader MK, et al. SARS-CoV-2 and RT-PCR testing in travelers: results of a cross-sectional study of travelers at Iraq's international borders. Disaster Med Public Health Prep. 2022;7: 1-3. doi: 10.1017/dmp.2022.140.
  12. Hussein NR, Rashad BH, Almizori LA, et al. The risk of SARS-CoV-2 reinfection in Duhok city, Kurdistan Region of Iraq. Mediterr J Hematol Infect Dis. 2021;13(1): e2021035. doi: 10.4084/MJHID.2021.035.
  13. World Health Organization Global surveillance for COVID-19 disease caused by human infection with the 2019 novel coronavirus. Interim guidance. 27 February 2020:2020.
  14. Singanayagam A, Patel M, Charlett A, et al. Duration of infectiousness and correlation with RT-PCR cycle threshold values in cases of COVID-19, England, January to May 2020. Euro Surveill. 2020;25(32):2001483. doi: 10.2807/1560-7917.ES.2020.25.32.2001483.
  15. Eskier D, Akalp E, Dalan Ö, et al. Current mutatome of SARS-CoV-2 in Turkey reveals mutations of interest. Turk J Biol. 2021 Feb 9;45(1):104-113. doi: 10.3906/biy-2008-56.
  16. Russell TW, Wu JT, Clifford S, et al. Effect of internationally imported cases on internal spread of COVID-19: a mathematical modelling study. Lancet Public Health. 2021;6(1): e12-e20. doi: 10.1016/S2468-2667(20)30263-2.
  17. Merza MA, Abdulah DM, Malo DH, et al. Current status of COVID-19 infection and vaccination coverage, and factors associated with infection acquisition and vaccine uptake. Infect Dis Trop Med. 2023;9: e1062. doi: 10.32113/idtm_20231_1062.


How to Cite

1.
Ghazwan Mohammed Raouf G, Qader M, Abdullah I, Younis O, Mohammad F, Merza M. Detection of SARS-CoV-2 variants among travelers crossing the northern international border checkpoint in Duhok province, Iraq . jidhealth [Internet]. 2023 Dec. 2 [cited 2024 Feb. 23];6(4):971-04. Available from: https://jidhealth.com/index.php/jidhealth/article/view/316