Abstract
Background: Leptospirosis is one of the most commonly neglected zoonoses in developing nations including Tanzania. This study aims to find out the seroprevalence of leptospirosis in rodents, shrews, and domestic animals in different regions in Unguja Island, Tanzania.
Methods: A cross-sectional study was carried out from January to April 2022. The blood samples were collected from rodents and shrews (n=248), cattle (n=247), goats (n=130), sheep (n=32), and dogs (n=80). The blood samples were allowed to clot in a slanted position and serum samples were harvested. A microscopic agglutination test (MAT) was performed on the sera to check for leptospiral antibodies using five Leptospira serovars as antigens (Sokoine, Lora, Pomona, Grippotyphosa and Hebdomadis).
Results: The overall seropositivity of leptospiral antibodies was 9.68% in rodents and shrews, 14.57% in cattle, 10.01% in goats, 31.25% in sheep, and 26.25% in dogs. The seropositivity of Leptospira varied significantly with animal species (OR=1.9, 95 % CI:1.1-3.3, p=0.03). The most frequently detected serovar was Sokoine (27.89%), followed by Pomona (19.47%), Lora (18.26%), Grippotyphosa (17.98%), and Hebdomadis (8.16%), respectively.
Conclusion: Our study suggests that further research should be conducted to find out factors of high seropositivity of leptospiral in Unguja. Vaccination of domestic animals with vaccines against local Leptospira strains should be encouraged, and rodent control and public awareness should be emphasized.
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References
- Motto SK, Shirima GM, de Clare Bronsvoort BM, Cook EAJ. Epidemiology of leptospirosis in Tanzania: A review of the current status, serogroup diversity and reservoirs. PLoS Negl Trop Dis. 2021 Nov 16;15(11):e0009918. doi: 10.1371/journal.pntd.0009918
- Mgode GF, Japhary MM, Mhamphi GG, Kiwelu I, Athaide I, Machang'u RS. Leptospirosis in sugarcane plantation and fishing communities in Kagera northwestern Tanzania. PLoS Negl Trop Dis. 2019 May 31;13(5):e0007225. doi: 10.1371/journal.pntd.0007225
- Torgerson PR, Hagan JE, Costa F, Calcagno J, Kane M, Martinez-Silveira MS, Goris MG, Stein C, Ko AI, Abela-Ridder B. Global Burden of Leptospirosis: Estimated in Terms of Disability Adjusted Life Years. PLoS Negl Trop Dis. 2015 Oct 2;9(10):e0004122. doi: 10.1371/journal.pntd.0004122
- Allan KJ, Biggs HM, Halliday JE, Kazwala RR, Maro VP, Cleaveland S, Crump JA. Epidemiology of Leptospirosis in Africa: A Systematic Review of a Neglected Zoonosis and a Paradigm for 'One Health' in Africa. PLoS Negl Trop Dis. 2015 Sep 14;9(9):e0003899. doi: 10.1371/journal.pntd.0003899. Erratum in: PLoS Negl Trop Dis. 2016 Mar;10(3):e0004552.
- Biggs HM, Hertz JT, Munishi OM, Galloway RL, Marks F, Saganda W, Maro VP, Crump JA. Estimating leptospirosis incidence using hospital-based surveillance and a population-based health care utilization survey in Tanzania. PLoS Negl Trop Dis. 2013 Dec 5;7(12):e2589. doi: 10.1371/journal.pntd.0002589.
- NBS. National Sample Census of Agriculture 2019/20, Key Findings Report for Crops and Livestock Sectors and Fish Farming.Dar es Salaam. Tanzania: National Bureau of Statistics. 2021 Aug 01. pg. 7(40). [https//www.nbs.go.tz › Tak › Agric ›PDF). 2021].
- ZLS. Annual Report, Department of Livestock Development Zanzibar, Tanzania. Zanzibar Livestock Survey 2021 Dec 01. Pg 95(181). https://www.ocgs.go.tz › php › ReportOCGS › PDF.2021].
- Machang’u RS, Mgode G, Mpanduji D. Leptospirosis in animals and humans in selected areas of Tanzania. Belgian Journal of Zoology. 1997;127 Suppl (January):97–104.
- Assenga JA, Matemba LE, Muller SK, Mhamphi GG, Kazwala RR. Predominant leptospiral serogroups circulating among humans, livestock and wildlife in Katavi-Rukwa ecosystem, Tanzania. PLoS Negl Trop Dis. 2015 Mar 25;9(3):e0003607. doi: 10.1371/journal.pntd. 0003607.
- Motto SK, Shirima GM, de Clare Bronsvoort BM, Cook EAJ. Epidemiology of leptospirosis in Tanzania: A review of the current status, serogroup diversity and reservoirs. PLoS Negl Trop Dis. 2021 Nov 16;15(11):e0009918. doi: 10.1371/journal.pntd.0009918.
- Msemwa B, Mirambo MM, Silago V, Samson JM, Majid KS, Mhamphi G, Genchwere J, Mwakabumbe SS, Mngumi EB, Mgode G, Mshana SE. Existence of Similar Leptospira Serovars among Dog Keepers and Their Respective Dogs in Mwanza, Tanzania, the Need for a One Health Approach to Control Measures. Pathogens. 2021 May 16;10(5):609. doi: 10.3390/pathogens10050609.
- Ally AA, Lupindu AM, Machang R, Katakweba AS. Seroprevalence of leptospirosis among hospitalized febrile patients in Unguja Island. 2023 march 27; 6(1):820–82. https://doi.org/10.47108/jidhealth.Vol6.Iss1.274
- Ali MA, James OC, Mohamed AA, Joachim A, Mubi M, Omodior O. Etiologic Agents of Fever of Unknown Origin Among Patients Attending Mnazi Mmoja Hospital, Zanzibar. J Community Health 2020 May 12;45(5):1073–80. https://doi.org/10.1007/s10900-020-00832-w.
- The United Republic of Tanzania (URT). Ministry of Finance and Planning, Tanzania National Bureau of Statistics and President’s Office - Finance and Planning, Office of the Chief Government Statistician, Zanzibar. The Population and Housing Census: Administrative Units Population Distribution Report; Tanzania, 2022 Dec 01. Vol1A: 1-290. [https://www.nbs.go.tz › takwimu › Census2022. PDF].
- The United Republic of Tanzania (URT). Ministry of Finance and Planning, Tanzania National Bureau of Statistics and President’s Office - Finance and Planning, Office of the Chief Government Statistician, Zanzibar. National Sample Census of Agriculture 2019/20 Atlas.2021, Aug 01. Pg 1-40. ttps://www.nbs.go.tz › takwimu›Agriculture.PDF
- Mlowe GD, Katakweba AS, Makundi I, Sabuni C. Assessment of Rodent Diversity and Abundance in different habitats of Unguja, Zanzibar, Tanzania. Sustain Biodivers Conserv 2023 Feb 12:2(1) 29–55: https://doi.org/10.5281/zenodo.7643678
- Mulungu LS, Makundi RH, Massawe AW, Machang’u RS, Mbije NE. Diversity and distribution of rodent and shrew species associated with variations in altitude on Mount Kilimanjaro, Tanzania. In: Mammalia. 2008 Oct 01. 72:(3); 178–85. https://doi.org/10.1515/MAMM.2008.021
- Mgode GF, Mhamphi GG, Massawe AW, Machang'u RS. Leptospira Seropositivity in Humans, Livestock and Wild Animals in a Semi-Arid Area of Tanzania. Pathogens. 2021 Jun 3;10(6):696. doi: 10.3390/pathogens10060696.
- Goris MG, Leeflang MM, Loden M, Wagenaar JF, Klatser PR, Hartskeerl RA, Boer KR. Prospective evaluation of three rapid diagnostic tests for diagnosis of human leptospirosis. PLoS Negl Trop Dis. 2013 Jul 11;7(7):e2290. doi: 10.1371/journal.pntd.0002290.
- Mgode GF, Machang'u RS, Mhamphi GG, Katakweba A, Mulungu LS, Durnez L, Leirs H, Hartskeerl RA, Belmain SR. Leptospira Serovars for Diagnosis of Leptospirosis in Humans and Animals in Africa: Common Leptospira Isolates and Reservoir Hosts. PLoS Negl Trop Dis. 2015 Dec 1;9(12):e0004251. doi: 10.1371/journal.pntd.0004251.
- Mirambo MM, Mgode GF, Malima ZO, John M, Mngumi EB, Mhamphi GG, Mshana SE. Seropositivity of Brucella spp. and Leptospira spp. antibodies among abattoir workers and meat vendors in the city of Mwanza, Tanzania: A call for one health approach control strategies. PLoS Negl Trop Dis. 2018 Jun 25;12(6):e0006600. doi: 10.1371/journal.pntd.0006600.
- Yupiana Y, Vallée E, Wilson P, Weston JF, Benschop J, Collins-Emerson J, Heuer C. On-farm risk factors associated with Leptospira shedding in New Zealand dairy cattle. Epidemiol Infect. 2020 May 18;148:e219. doi: 10.1017/S095026882000103X
- Swai ES, Schoonman L. A survey of zoonotic diseases in trade cattle slaughtered at Tanga city abattoir: a cause of public health concern. Asian Pac J Trop Biomed. 2012 Jan;2(1):55-60. doi: 10.1016/S2221-1691(11)60190-1
- Karimuribo ED, Swai ES, Kyakaisho PK. Investigation of a syndrome characterised by passage of red urine in smallholder dairy cattle in East Usambara Mountains, Tanzania. J S Afr Vet Assoc. 2008 Jun;79(2):89-94. doi: 10.4102/jsava.v79i2.250.
- Mgode GF, Katakweba AS, Mhamphi GG, Fwalo F, Bahari M, Mdangi M, Kilonzo BS, Mulungu LS. Prevalence of leptospirosis and toxoplasmosis: a study of rodents and shrews in cultivated and fallow land, Morogoro rural district, Tanzania. Tanzan J Health Res. 2014 Jul;16(3):250-5. doi: 10.4314/thrb.v16i3.11.
- Katakweba A. Small Mammals in Fenced Houses as Source of Leptospirosis to Livestock Pets animals and Humans in Morogoro Municipality, Tanzania. Tanzania Veterinary Association Proceedings [Internet]. 2018;36(2018). Available from: https://www.ajol.info/index.php/tvj/article/view/194951%0Ahttps://tvj1.sua.ac.tz/index.php/TVJ/article/view/83
- Machang'u RS, Mgode GF, Assenga J, Mhamphi G, Weetjens B, Cox C, Verhagen R, Sondij S, Goris MG, Hartskeerl RA. Serological and molecular characterization of leptospira serovar Kenya from captive African giant pouched rats (Cricetomys gambianus) from Morogoro Tanzania. FEMS Immunol Med Microbiol. 2004 Jun 1;41(2):117-21. doi: 10.1016/j.femsim.2004.02.002.
- Ahmed N, Devi SM, Valverde Mde L, Vijayachari P, Machang'u RS, Ellis WA, Hartskeerl RA. Multilocus sequence typing method for identification and genotypic classification of pathogenic Leptospira species. Ann Clin Microbiol Antimicrob. 2006 Nov 23;5:28. doi: 10.1186/1476-0711-5-28.
- Allan KJ, Bvm H. Leptospirosis in northern Tanzania : investigating the role of rodents and ruminant livestock in a neglected public health problem. PhD thesis. University of Glasgow. 2016 June 01. Pg. 1-299. http://theses.gla.ac.uk/7565/
- Okewole EA, Ayoola MO. Seroprevalence of leptospiral serovars other than Canicola and Icterohaemorrhagiae in dogs in the Southwestern Nigeria. Veterinarski arhiv. 2009 Feb 24;79(1):87-96.
- Haji Hajikolaei MR, Rezaei S, Ghadrdan Mashhadi AR, Ghorbanpoor M. The role of small ruminants in the epidemiology of leptospirosis. Sci Rep. 2022 Feb 9;12(1):2148. doi: 10.1038/s41598-022-05767-x.
- Haake DA, Levett PN. Leptospirosis in humans. Leptospira and leptospirosis. Curr Top Microbiol Immunol, 2015 Nov 12:65-97.
